A standardized laboratory and surgical method for in vitro culture isolation and expansion of primary human Tenon’s fibroblasts

@article{Falco2012ASL,
  title={A standardized laboratory and surgical method for in vitro culture isolation and expansion of primary human Tenon’s fibroblasts},
  author={Elena De Falco and Gaia Scafetta and Chiara Napoletano and Rosa Puca and Enzo Maria Vingolo and Giuseppe Ragona and Olga Iorio and Giacomo Frati},
  journal={Cell and Tissue Banking},
  year={2012},
  volume={14},
  pages={277-287}
}
Good manufacturing practices guidelines require safer and standardized cell substrates especially for those cell therapy products to treat ocular diseases where fibroblasts are used as feeder layers. However, if these are unavailable for stem cells culturing, murine fibroblasts are regularly used, raising critical issues as accidentally transplanting xenogenous graft and adversely affecting stem cell clinical trials. Moreover, human fibroblasts play a significant role in testing novel… CONTINUE READING

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