A standard addition method to assay the concentration of biologically interesting polyphenols in grape berries by reversed-phase HPLC.


A reversed-phase HPLC method which allows the simultaneous assay of (+)- catechin, (-)-epicatechin, trans-resveratrol, quercetin and quercetin glycosides in grape berries is described. Kromasil 100 served as stationary phase and a gradient of acetic acid, water and methanol was used. The analytical run requires 42 min for complete sample elution… (More)


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