A spontaneous murine melanoma lung metastasis comprised of host x tumor hybrids.

Abstract

Cells from a lung metastasis, arising from Cloudman S91 melanoma cells implanted s.c. in the tail of a BALB/c nu/nu mouse, were comprised chiefly of host x tumor hybrids. These lung metastasis cells showed: (a) 30-40% increased DNA content; (b) resistance to 10(-4) M hypoxanthine, 4 x 10(-7) M aminopterin, and 1.6 x 10(-5) M thymidine (HAT) + G418; and (c) the presence in genomic DNA of genes for both wt and albino tyrosinase, reflecting the DBA/2J (Cloudman S91) and BALB/c mouse genotypes, respectively. Individual clones of lung metastasis cells expressed enhanced pigmentation, motility, and responsiveness to MSH/IBMX, a behavior similar to that recently reported for artificially generated melanoma x macrophage fusion hybrids. These similarities suggested that the host fusion partner generating the lung metastasis hybrids might have been a macrophage, although formal proof for this was not possible. The results provide the first direct evidence that host x tumor hybridization could serve as an initiating mechanism for melanoma metastasis.

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@article{Chakraborty2000ASM, title={A spontaneous murine melanoma lung metastasis comprised of host x tumor hybrids.}, author={A K Chakraborty and S Sodi and M Rachkovsky and N Kolesnikova and J T Platt and J L Bolognia and J M Pawelek}, journal={Cancer research}, year={2000}, volume={60 9}, pages={2512-9} }