A split intein T7 RNA polymerase for transcriptional AND-logic

@inproceedings{Schaerli2014ASI,
  title={A split intein T7 RNA polymerase for transcriptional AND-logic},
  author={Yolanda Schaerli and Mag{\"u}i Gili and Mark Isalan},
  booktitle={Nucleic acids research},
  year={2014}
}
Synthetic biology has developed numerous parts for building synthetic gene circuits. However, few parts have been described for prokaryotes to integrate two signals at a promoter in an AND fashion, i.e. the promoter is only activated in the presence of both signals. Here we present a new part for this function: a split intein T7 RNA polymerase. We divide T7 RNA polymerase into two expression domains and fuse each to a split intein. Only when both domains are expressed does the split intein… CONTINUE READING
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