A spindle checkpoint arrest and a cytokinesis failure by the dominant-negative polo-box domain of Plk1 in U-2 OS cells.

@article{Seong2002ASC,
  title={A spindle checkpoint arrest and a cytokinesis failure by the dominant-negative polo-box domain of Plk1 in U-2 OS cells.},
  author={Yeon-Sun Seong and Keiju Kamijo and Jae-Seon Lee and Ester Corbacho Fern{\'a}ndez and Ryoko Kuriyama and Toru Miki and Kyung S Lee},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 35},
  pages={32282-93}
}
Polo kinases play critical roles for proper M-phase progression. They are characterized by the presence of two regions of homology in the C-terminal non-catalytic domain, termed polo-box 1 (PB1) and polo-box 2 (PB2). Here we demonstrate that both PB1 and PB2 are required for targeting the catalytic activity of Plk1 to centrosomes, midbody, and kinetochores. Expression of either kinase-inactive PLK1/K82M or the C-terminal plk1 Delta N induced a pre-anaphase arrest with elevated Cdc2 and Plk1… CONTINUE READING

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