A spectroscopic characterization of a monomeric analog of copper, zinc superoxide dismutase

@article{Bertini1994ASC,
  title={A spectroscopic characterization of a monomeric analog of copper, zinc superoxide dismutase},
  author={Ivano Bertini and Mario Piccioli and Maria Silvia Viezzoli and Choi Ying Chiu and G. T. Mullenbach},
  journal={European Biophysics Journal},
  year={1994},
  volume={23},
  pages={167-176}
}
A mutated protein of human Cu(II)2Zn(II)2 SOD in which residues Phe50 and Gly51 at the dimer interface were substituted by Glu's, thus producing a monomeric species, has been characterized by electronic absorption spectroscopy, EPR, relaxivity and1H NMR techniques. Such substitutions and/or accompanying remodeling and exposure of the dimer interface to solvent, alter the geometry of the active site: increases in the axiality of the copper chromophore and the Cu-OH2 distance have been observed… CONTINUE READING
Highly Cited
This paper has 26 citations. REVIEW CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 56 references

An investigation of a human erythrocyte SOD modified at the position 137

  • I Bertini, L Banci, +4 authors RA Hallewell
  • Inorg Chem
  • 1989
Highly Influential
6 Excerpts

Copper zinc superoxide dismutase: a paramagnetic protein that provides a unique frame for the NMR investigations

  • I Bertini, C Luchinat, M Piccioli
  • Progr Nucl Magn Reson Spectrosc
  • 1994
1 Excerpt

1 D versus 2 D 1 H NMR experiments in dicopper , dicobalt superoxide dismutase : a further mapping of the active site

  • L Banci, I Bertini, C Luchinat, M Piccioli, A Scozzafava
  • Gazz Chim Ital
  • 1993

Molecular dynamics studies on superoxide dismutase and its mutants : the structural and functional role of Arg 143

  • N Niccolai, P Carloni, G LaPenna, PL Orioli
  • J Am Chem Soc
  • 1992

1991 b) Role of Arg-143 in human Cu2Zn2SOD studied through anion binding

  • I Bertini, A Lepori, C Luchinat, P Turano
  • Inorg Chem
  • 1991

Similar Papers

Loading similar papers…