A spectrophotometric assay for alpha-ketoaldehydes using horse liver alcohol dehydrogenase.

Abstract

alpha-Ketoaldehydes have been extensively employed as reagents for the chemical modification of arginine residues in proteins, and to probe for putative anion recognition sites. A major disadvantage in their use is instability, as alpha-ketoaldehydes are prone to hydration, oxidation, and polymerization. These reagents are typically supplied as the aldehyde… (More)

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