A specific targeting signal directs Runx2/Cbfa1 to subnuclear domains and contributes to transactivation of the osteocalcin gene.

@article{Zaidi2001AST,
  title={A specific targeting signal directs Runx2/Cbfa1 to subnuclear domains and contributes to transactivation of the osteocalcin gene.},
  author={Sayyed K Zaidi and Ameera Javed and J Y Choi and Andre J van Wijnen and Janet L Stein and Jane B Lian and Gary S Stein},
  journal={Journal of cell science},
  year={2001},
  volume={114 Pt 17},
  pages={3093-102}
}
Key components of DNA replication and the basal transcriptional machinery as well as several tissue-specific transcription factors are compartmentalized in specialized nuclear domains. In the present study, we show that determinants of subnuclear targeting of the bone-related Runx2/Cbfa1 protein reside in the C-terminus. With a panel of C-terminal mutations, we further demonstrate that targeting of Runx2 to discrete subnuclear foci is mediated by a 38 amino acid sequence (aa 397-434). This… CONTINUE READING