A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast.

@article{Eisenkolb2002ASS,
  title={A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast.},
  author={Marlis Eisenkolb and Christoph Zenzmaier and E. von Leitner and Roger Schneiter},
  journal={Molecular biology of the cell},
  year={2002},
  volume={13 12},
  pages={
          4414-28
        }
}
Fungal sphingolipids contain ceramide with a very-long-chain fatty acid (C26). To investigate the physiological significance of the C26-substitution on this lipid, we performed a screen for mutants that are synthetically lethal with ELO3. Elo3p is a component of the ER-associated fatty acid elongase and is required for the final elongation cycle to produce C26 from C22/C24 fatty acids. elo3delta mutant cells thus contain C22/C24- instead of the natural C26-substituted ceramide. We now report… CONTINUE READING

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