A specific radioimmunoassay for estrone sulfate in plasma and urine without hydrolysis.

  title={A specific radioimmunoassay for estrone sulfate in plasma and urine without hydrolysis.},
  author={Keith Wright and Delwood C. Collins and Paul I Musey and J R Preedy},
  journal={The Journal of clinical endocrinology and metabolism},
  volume={47 5},
Estrone sulfate, quantitatively the most important estrogen in plasma, has previously been determined only after hydrolysis and chromatography. An antiserum raised against estrone glucosiduronate-bovine thyroglobulin was found to be suitable for the specific RIA of estrone sulfate both in plasma and urine. Plasma levels were measured after solvent extraction without hydrolysis or chromatography. The mean (+/-SE) was 972 +/- 79 pg/ml (range, 537-1581) in 15 women in the follicular phase, 1806… CONTINUE READING


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