A specific mutation abolishing Na+/H+ antiport activity in hamster fibroblasts precludes growth at neutral and acidic pH.

@article{Pouyssegur1984ASM,
  title={A specific mutation abolishing Na+/H+ antiport activity in hamster fibroblasts precludes growth at neutral and acidic pH.},
  author={Jacques M. Pouyssegur and Claude Sardet and Arlette Franchi and Gilles L'Allemain and Sonia Paris},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 15},
  pages={4833-7}
}
A H+-suicide technique based on the reversibility of Na+/H+ antiport was developed for the selection of mutants deficient in this membrane-bound activity. The strategy was to use the Na+/H+ antiporter as a H+-vector killing device. Chinese hamster lung fibroblasts (CCL39) were loaded with LiCl and incubated in Na+-, Li+-free choline Cl saline solution (pH 5.5). Under these conditions, intracellular pH dropped in 5 min from 7.1 to 4.8, leading to a rapid loss of cell viability (less than 0.1… CONTINUE READING
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