A specific immunoassay for monitoring human bone resorption: quantitation of type I collagen cross-linked N-telopeptides in urine.

@article{Hanson1992ASI,
  title={A specific immunoassay for monitoring human bone resorption: quantitation of type I collagen cross-linked N-telopeptides in urine.},
  author={Dennis A. Hanson and Mary Ann Weis and A M Bollen and Susan Maslan and Frederick R. Singer and David R Eyre},
  journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
  year={1992},
  volume={7 11},
  pages={1251-8}
}
Peptides of low molecular weight that contain pyridinoline cross-links were isolated from adolescent human urine. A fraction was selected that was enriched in the N-telopeptide-to-helix intermolecular cross-linking domain of bone type I collagen. Mouse monoclonal antibodies were generated against these urinary peptides conjugated to a carrier protein as immunogen. A high-affinity antibody was identified that specifically bound to the trivalent peptides derived from the N-telopeptide-to-helix… CONTINUE READING