A specific and versatile genome walking technique.

@article{Guo2006ASA,
  title={A specific and versatile genome walking technique.},
  author={Haitao Guo and Jin Feng Xiong},
  journal={Gene},
  year={2006},
  volume={381},
  pages={18-23}
}
We describe here a nested PCR-based strategy for genome walking to extend a known sequence region to its uncharacterized flanking regions. This technique involves the use of a partially degenerate primer as a walker primer and a set of nested specific primers to perform two to three successive rounds of nested PCR. To increase the success rate of genome walking, four different walker primers were designed to allow the setup of parallel reactions. This technique was applied to amplify flanking… CONTINUE READING

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