A spatial gradient coordinates cell size and mitotic entry in fission yeast

@article{Moseley2009ASG,
  title={A spatial gradient coordinates cell size and mitotic entry in fission yeast},
  author={James B. Moseley and Adeline Mayeux and Anne Paoletti and Paul Nurse},
  journal={Nature},
  year={2009},
  volume={459},
  pages={857-860}
}
Many eukaryotic cell types undergo size-dependent cell cycle transitions controlled by the ubiquitous cyclin-dependent kinase Cdk1 (refs 1–4). The proteins that control Cdk1 activity are well described but their links with mechanisms monitoring cell size remain elusive. In the fission yeast Schizosaccharomyces pombe, cells enter mitosis and divide at a defined and reproducible size owing to the regulated activity of Cdk1 (refs 2, 3). Here we show that the cell polarity protein kinase Pom1… 
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Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry
TLDR
This work reveals how Pom1-Cdr2-Wee1 operates in multiprotein clusters at the cell cortex to promote mitotic entry at a specific size that can be modified by nutrient availability.
Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry
TLDR
This work reveals how Pom1-Cdr2-Wee1 operates in multiprotein clusters at the cortex to promote mitotic entry at a cell size that can be modified by nutrient availability.
Dueling Kinases Regulate Cell Size at Division through the SAD Kinase Cdr2
Cyclin-Dependent Kinase 8 Regulates Mitotic Commitment in Fission Yeast
TLDR
It is reported that Mediator, a conserved coregulator of eukaryotic transcription, is part of a regulatory pathway that controls mitotic entry in fission yeast and establishes a new pathway linking the Mediator complex to control of mitotic transcription and regulation of mitosis in fissions yeast.
Dynamic regulation of Cdr1 kinase localization and phosphorylation during osmotic stress
TLDR
A mechanism that prevents Cdr1 colocalization with its inhibitory target Wee1 during osmotic stress is identified and dynamic regulation of protein localization to cortical nodes might represent a strategy to modulate entry into mitosis under differing environmental conditions.
A quantitative and spatial analysis of cell cycle regulators during the fission yeast cycle
TLDR
The mitotic B-type cyclin Cdc13 and mitotic inhibitory phosphatase Cdc25 are identified as the only two proteins that change in both whole cell and nuclear concentration through the cell cycle, making them candidates for universal cell size sensors at the onset of mitosis and cell division.
Cell-size control
TLDR
This work evaluated the Pom1 gradient model on cell-size control and provided 3 lines of evidence to show that partial loss of Pom1 protein level or activity, while not affecting division-site positioning, displays premature mitotic entry.
Cell size–dependent regulation of Wee1 localization by Cdr2 cortical nodes
TLDR
It is found that increasing Cdr2 activity during cell growth promotes Wee1 localization to nodes, where inhibitory phosphorylation of Wee1 by Cdr1 and C dr2 kinases promotes mitotic entry.
Cell size-dependent regulation of Wee1 localization by Cdr2 cortical nodes
TLDR
This study shows how cell growth progressively inhibits fission yeast Wee1 through dynamic bursts of localization to cortical node structures that contain Wee1 inhibitory kinases.
A Phosphorylation Cycle Shapes Gradients of the DYRK Family Kinase Pom1 at the Plasma Membrane
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References

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TLDR
It is shown that Pom1p kinase activity is cell cycle regulated in correlation with the state of cellular symmetry: the activity is high during symmetrical growth and division, but lower when cells grow at just one end, and there may be related roles of homologous protein kinases ubiquitously present in all eukaryotes.
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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