A soybean sucrose binding protein independently mediates nonsaturable sucrose uptake in yeast.

@article{Overvoorde1996ASS,
  title={A soybean sucrose binding protein independently mediates nonsaturable sucrose uptake in yeast.},
  author={Paul J Overvoorde and Wolf Bernd Frommer and Howard Dean Grimes},
  journal={The Plant cell},
  year={1996},
  volume={8 2},
  pages={271-80}
}
Heterologous expression of a cDNA encoding a 62-kD soybean sucrose binding protein in yeast demonstrates that this protein, independent of other plant proteins, mediates sucrose uptake across the plasma membrane. Sucrose binding protein-mediated sucrose uptake is nonsaturable up to 30 mM sucrose, is specific for sucrose, and is relatively insensitive to treatment with sulfhydryl-modifying reagents. Alteration of the external pH or pretreatment of the yeast cells with protonophores did not… CONTINUE READING

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