A solvent-free method for spiking terrestrial algae (Desmococcus spp.) with pyrene for use in bioassays.

@article{Howsam2003ASM,
  title={A solvent-free method for spiking terrestrial algae (Desmococcus spp.) with pyrene for use in bioassays.},
  author={Mike Howsam and Rudo A. Verweij and Nico M van Straalen},
  journal={Chemosphere},
  year={2003},
  volume={53 3},
  pages={253-62}
}
Two methods for spiking terrestrial algae (Desmococcus spp., used as a foodstuff in bioassays with Collembola) with pyrene were tested; a "traditional" method that used a carrier solution of pyrene in acetone, and a solvent-free method developed using the principles of partition driven administration (PDA). The PDA method used a pre-spiked, highly-contaminated C18 disk as the source for pyrene, suspended and rotated in an aqueous algal suspension. The pyrene partitioned from the C18 disk into… CONTINUE READING