A solid-phase immobilized epitope immunoassay (SPIE-IA) permitting very sensitive and specific measurement of angiotensin II in plasma.

@article{Volland1999ASI,
  title={A solid-phase immobilized epitope immunoassay (SPIE-IA) permitting very sensitive and specific measurement of angiotensin II in plasma.},
  author={Herv{\'e} Volland and Philippe Pradelles and Pierre Ronco and Michel Azizi and Dan Simon and Christophe A Cr{\'e}minon and J acques Grassi},
  journal={Journal of immunological methods},
  year={1999},
  volume={228 1-2},
  pages={37-47}
}
We have developed a new enzyme immunometric assay for angiotensin II (AII) based on SPIE-IA technology (solid-phase immobilized epitope-immunoassay). A monoclonal antibody with optimal properties (mAb3 131) was selected from a series of 19 anti-AII mAbs. The mAb had to be purified from ascitic fluid in a specific manner in order to remove endogenous AII from the antibody-binding sites. We established a sensitive (minimum detectable concentration 0.5 pg/ml) and precise (CV below 15% in the 2-100… CONTINUE READING

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