A site-specific DNA endonuclease specified by one of two ORFs encoded by a group I intron in Dictyostelium discoideum mitochondrial DNA.

@article{Ogawa1997ASD,
  title={A site-specific DNA endonuclease specified by one of two ORFs encoded by a group I intron in Dictyostelium discoideum mitochondrial DNA.},
  author={Sonoko Ogawa and Kimitoshi Naito and Kiyohiko Angata and Takahiro Morio and Hideko Urushihara and Yoshimasa Tanaka},
  journal={Gene},
  year={1997},
  volume={191 1},
  pages={115-21}
}
The second intron (DdOX1/2.2) of Dictyostelium discoideum cytochrome oxidase subunit 1/2 fused gene has two free-standing ORF genes (Dd ai2a and Dd ai2b) in a loop, which have similar amino acid sequences and are homologous to aI4 DNA endonuclease (I-SceII) of Saccharomyces cerevisiae. To elucidate the functions of these ORFs, we cloned the ORFs into an expression vector and introduced the composite vectors into E. coli. The expression of Dd ai2a in E. coli caused growth inhibition and… CONTINUE READING