A site-directed mutagenesis study of yeast calmodulin.

  title={A site-directed mutagenesis study of yeast calmodulin.},
  author={Isao Matsuura and Katsutoshi Ishihara and Yoshihiko Nakai and Michio Yazawa and H K Toda and Kunio Yagi},
  journal={Journal of biochemistry},
  volume={109 1},
A site-directed mutagenesis study was carried out in order to understand the regulatory mechanism of calmodulin. We started from the yeast (Saccharomyces cerevisiae) calmodulin gene since it has many differences in amino acid sequence and inferior functional properties compared with the vertebrate calmodulin. Recombinant yeast calmodulins were generated in Escherichia coli transformed by constructed expression plasmids. Three recombinant calmodulins were obtained. The first two were YCM61G, in… CONTINUE READING

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