A single-stranded DNA binding protein binds the origin of replication of the duplex kinetoplast DNA.

@article{Avrahami1995ASD,
  title={A single-stranded DNA binding protein binds the origin of replication of the duplex kinetoplast DNA.},
  author={Dana Avrahami and Yehuda Tzfati and Joseph Shlomai},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 23},
  pages={10511-5}
}
Replication of the kinetoplast DNA (kDNA) minicircle of trypanosomatids initiates at a conserved 12-nt sequence, 5'-GGGGTTGGTGTA-3', termed the universal minicircle sequence (UMS). A sequence-specific single-stranded DNA-binding protein from Crithidia fasciculata binds the heavy strand of the 12-mer UMS. Whereas this UMS-binding protein (UMSBP) does not bind a duplex UMS dodecamer, it binds the double-stranded kDNA minicircle as well as a duplex minicircle fragment containing the origin… CONTINUE READING