A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides.

@article{Tabor1995ASR,
  title={A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides.},
  author={Stanley Tabor and Charles. Richardson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 14},
  pages={
          6339-43
        }
}
  • Stanley Tabor, Charles. Richardson
  • Published in
    Proceedings of the National…
    1995
  • Biology, Medicine
  • Bacteriophage T7 DNA polymerase efficiently incorporates a chain-terminating dideoxynucleotide into DNA, in contrast to the DNA polymerases from Escherichia coli and Thermus aquaticus. The molecular basis for this difference has been determined by constructing active site hybrids of these polymerases. A single hydroxyl group on the polypeptide chain is critical for selectivity. Replacing tyrosine-526 of T7 DNA polymerase with phenylalanine increases discrimination against the four… CONTINUE READING

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