A single polypeptide catalyzing the conversion of phytoene to zeta-carotene is transcriptionally regulated during tomato fruit ripening.

@article{Pecker1992ASP,
  title={A single polypeptide catalyzing the conversion of phytoene to zeta-carotene is transcriptionally regulated during tomato fruit ripening.},
  author={Iris Pecker and David Chamovitz and Hartmut Linden and G erhard Sandmann and Joseph Hirschberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 11},
  pages={
          4962-6
        }
}
The cDNA of the gene pds from tomato, encoding the carotenoid biosynthesis enzyme phytoene desaturase, was cloned, and its nucleotide sequence was determined. Cells of Escherichia coli that expressed the tomato pds gene could convert phytoene to zeta-carotene. This result suggests that one polypeptide, the product of the pds gene, can carry out phytoene desaturation in the carotenoid biosynthetic pathway. Transcripts of the pds gene accumulate in orange tomato fruit, indicating transcriptional… CONTINUE READING
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