A single peptide-major histocompatibility complex ligand triggers digital cytokine secretion in CD4(+) T cells.

@article{Huang2013ASP,
  title={A single peptide-major histocompatibility complex ligand triggers digital cytokine secretion in CD4(+) T cells.},
  author={Jun Huang and Mario Brameshuber and Xun Zeng and Jianming Xie and Qi-jing Li and Yueh-hsiu Chien and Salvatore Valitutti and Mark M. Davis},
  journal={Immunity},
  year={2013},
  volume={39 5},
  pages={846-57}
}
We have developed a single-molecule imaging technique that uses quantum-dot-labeled peptide-major histocompatibility complex (pMHC) ligands to study CD4(+) T cell functional sensitivity. We found that naive T cells, T cell blasts, and memory T cells could all be triggered by a single pMHC to secrete tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) cytokines with a rate of ∼1,000, ∼10,000, and ∼10,000 molecules/min, respectively, and that additional pMHCs did not augment secretion… CONTINUE READING
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