A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding.

@article{Amblar2005ASM,
  title={A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding.},
  author={M{\'o}nica Amblar and Cec{\'i}lia M Arraiano},
  journal={The FEBS journal},
  year={2005},
  volume={272 2},
  pages={363-74}
}
Exoribonuclease II (RNase II), encoded by the rnb gene, is a ubiquitous enzyme that is responsible for 90% of the hydrolytic activity in Escherichia coli crude extracts. The E. coli strain SK4803, carrying the mutant allele rnb296, has been widely used in the study of the role of RNase II. We determined the DNA sequence of rnb296 and cloned this mutant gene in an expression vector. Only a point mutation in the coding sequence of the gene was detected, which results in the single substitution of… CONTINUE READING
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