A single gene and a pseudogene for the cellular tumour antigen p53

@article{ZakutHouri1983ASG,
  title={A single gene and a pseudogene for the cellular tumour antigen p53},
  author={R Zakut-Houri and Moshe Oren and B Bienz and Vered Lavie and Shulamith Hazum and David Givol},
  journal={Nature},
  year={1983},
  volume={306},
  pages={594-597}
}
The cellular tumour antigen p53 is a protein found in elevated levels in a great variety of transformed cells (reviewed in ref. 1). Overproduction of p53 was observed in cells transformed by a wide spectrum of agents2–7 as well as in embryonal carcinoma cells3,8 and in spontaneous transformants9,10. Although initially described in mice2,3,9, similar p53-like proteins were also observed in cells of other species, including those derived from several human tumours11. In non-transformed cells the… 
Participation of p53 cellular tumour antigen in transformation of normal embryonic cells
TLDR
It is demonstrated here that p53 can cooperate with the activated Ha-ras oncogene to transform normal embryonic cells, and the resultant foci contain cells of a markedly altered morphology which produce high levels of p53.
Overproduction of p53 antigen makes established cells highly tumorigenic
TLDR
It is demonstrated here that overproduction of p53 in an established cell line, while not causing gross morphological changes, endows these cells with an overt tumorigenic potential, and the tumorigenics efficiency of such cell lines may be correlated with the extent of p 53 overproduction.
Rearrangements of the cellular p53 gene in erythroleukaemic cells transformed by Friend virus
TLDR
It is demonstrated that genomic rearrangements are responsible for p53 gene Jnactivation in these cell lines and that they occur in vivo during the natural progression of Friend virus-induced erythroleukaemia.
p53 and human malignancies.
Modulation of p53 function by adenovirus E1B58kDa
TLDR
Results from this thesis have indicated that ElB58kDa can modulate some biological activities of wild type p53, but which ones depends on the biological system being studied.
Stabilization of the p53 transformation-related protein in mouse fibrosarcoma cell lines: effects of protein sequence and intracellular environment
TLDR
The results indicated that the primary structure of the p53 protein is a major determinant of its turnover rate; different p53 variants were degraded at distinct and characteristic rates in a number of transformed cell types.
[P53 tumor suppressor gene].
Molecular basis for heterogeneity of the human p53 protein
TLDR
Exchanging the 5' fragments of interest and expressing the chimeric clones in vitro confirmed that the DNA heterogeneity was responsible for the difference in the electrophoretic mobility of these proteins.
Deletion of 5'-coding sequences of the cellular p53 gene in mouse erythroleukemia: a novel mechanism of oncogene regulation
TLDR
This study represents the first description of an altered p53 gene product arising by mutation during neoplastic progression and identifies a region in the p53 protein molecule that plays a role in determining p53 stability in vivo.
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References

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Regulation of the cellular p53 tumor antigen in teratocarcinoma cells and their differentiated progeny.
TLDR
The results demonstrate that the high levels of p53 in F9 cells relative to their differentiated progeny were not due to alterations in the stability or turnover of this protein, and the regulation during differentiation involved a marked decrease in the amount of in vitro translatable p53 mRNA detected in the differentiated cell cultures.
p53 transformation-related protein: detection by monoclonal antibody in mouse and human cells.
TLDR
The results suggest that p53 may be involved in the normal regulation of cell division and that malignant transformation leads to abnormalities in the control of p53 expression.
Purification and partial amino acid sequence analysis of the cellular tumour antigen, p53, from mouse SV40‐transformed cells.
TLDR
High sensitivity automated gas‐phase sequencing techniques are used to determine the amino acid sequence of two tryptic peptides from p53, which agree completely with the predicted polypeptide sequence derived from a cloned cDNA for p53 mRNA and provide the first data on the amino acids sequence of p53.
Detection of a common feature in several human tumor cell lines--a 53,000-dalton protein.
TLDR
Human cell lines, whether derived from spontaneous tumors or transformed in vitro with simian virus 40, were found to contain a 53,000-dalton phosphoprotein (pp53) in contrast to normal human cells in which this protein was not detected, and it is suggested that this protein may be associated with their transformed state.
Post-translational regulation of the 54K cellular tumor antigen in normal and transformed cells.
TLDR
A post-translational regulation of the 54K cellular tumor antigen is demonstrated and it is suggested that this control is mediated by the SV40 large T-antigen.
Analysis of recombinant DNA clones specific for the murine p53 cellular tumor antigen.
TLDR
Three cDNA clones, corresponding to two non‐overlapping regions of the mRNA coding for the mouse p53 cellular tumor antigen, were isolated and characterized and evidence was found for the existence of two distinct p53‐specific genes in mouse genomic DNA.
Quantitation of a 55K cellular protein: similar amount and instability in normal and malignant mouse cells.
TLDR
In primary cells established from the tumors induced by highly tumorigenic cells there was a very low or no detectable amount of the 55K protein, in contrast to the primary cells obtained from early murine embryos in which the authors have reported high amounts of (stable) 55K proteins.
Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse.
TLDR
The presence of p53 in tumors of no known viral etiology indicates coding by resident cellular genes; this does not exclude endogenous viruses as the source of coding sequences or the possibility that transforming viruses code directly for p53.
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