A single extraction and HPLC procedure for simultaneous analysis of phytosterols, tocopherols and lutein in soybeans.

@article{Slavin2012ASE,
  title={A single extraction and HPLC procedure for simultaneous analysis of phytosterols, tocopherols and lutein in soybeans.},
  author={Margaret Slavin and Liangli Lucy Yu},
  journal={Food chemistry},
  year={2012},
  volume={135 4},
  pages={2789-95}
}
A saponification/extraction procedure and high performance liquid chromatography (HPLC) analysis method were developed and validated for simultaneous analysis of phytosterols, tocopherols and lutein (a carotenoid) in soybeans. Separation was achieved on a phenyl column with a ternary, isocratic solvent system of acetonitrile, methanol and water (48:22.5:29.5, v/v/v). Evaporative light scattering detection (ELSD) was used to quantify β-sitosterol, stigmasterol, campesterol, and α-, δ- and… CONTINUE READING
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