A single copy of SecYEG is sufficient for preprotein translocation.

  title={A single copy of SecYEG is sufficient for preprotein translocation.},
  author={Alexej Kedrov and Ilja Kusters and Victor V. Krasnikov and Arnold J M Driessen},
  journal={The EMBO journal},
  volume={30 21},
The heterotrimeric SecYEG complex comprises a protein-conducting channel in the bacterial cytoplasmic membrane. SecYEG functions together with the motor protein SecA in preprotein translocation. Here, we have addressed the functional oligomeric state of SecYEG when actively engaged in preprotein translocation. We reconstituted functional SecYEG complexes labelled with fluorescent markers into giant unilamellar vesicles at a natively low density. Förster's resonance energy transfer and… CONTINUE READING
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Kinetic analysis of the translocation of fluorescent precursor proteins into Escherichia coli membrane vesicles

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