A single-chain H-2Db molecule presenting an influenza virus nucleoprotein epitope shows enhanced ability at stimulating CD8+ T cell responses in vivo.

@article{Palmowski2009ASH,
  title={A single-chain H-2Db molecule presenting an influenza virus nucleoprotein epitope shows enhanced ability at stimulating CD8+ T cell responses in vivo.},
  author={Michael J. Palmowski and Mathew Parker and Kaushik Choudhuri and Christopher Chiu and Margaret F. C. Callan and Philip Anton van der Merwe and Vincenzo Cerundolo and Keith G. Gould},
  journal={Journal of immunology},
  year={2009},
  volume={182 8},
  pages={4565-71}
}
We have generated a construct encoding a single-chain H-2D(b) mouse MHC class I molecule in which an influenza virus nucleoprotein (NP) epitope, amino acid sequence ASNENMDAM, is fused to mouse beta(2)-microglobulin and the D(b) H chain via flexible linker sequences. This single-chain trimer (SCT) was efficiently expressed at the cell surface independently of TAP and endogenous beta(2)-microglobulin, and it was recognized directly and efficiently by specific T cells in vitro. A recombinant… CONTINUE READING
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