A single bone marrow-derived stromal cell type supports the in vitro growth of early lymphoid and myeloid cells

  title={A single bone marrow-derived stromal cell type supports the in vitro growth of early lymphoid and myeloid cells},
  author={Pamela Hunt and D Robertson and Diane Weiss and Donna M. Rennick and Frank S. Lee and Owen N. Witte},
Differentiation of Human Mast Cells from Bone‐Marrow and Cord‐Blood Progenitor Cells by Factors Produced by a Mouse Stromal Cell Line
The culture of immunomagnetically purified human-bone-marrow- or cord-blood-derived CD34+ cells on a supportive mouse lipoblastic stromal cell line, MS-5, allows for significant amounts of highly enriched normal human mast cell populations useful for further studies on the reactivity of this cell subset.
Long-term survival of human myeloid progenitor cells induced by a mouse bone marrow stromal cell line.
Mouse endothelial-adipocyte cell line (14F1.1), which induces proliferation of mouse stem cells in culture, is also capable of supporting long-term survival in culture of human myeloid progenitor
Characterization of murine bone marrow and spleen-derived stromal cells: analysis of leukocyte marker and growth factor mRNA transcript levels.
Results indicate that although stromal cells appear to be mesodermal in origin, they are not closely related developmentally to the hematopoietic progenitor cells they support, and suggest that they are a multifunctional component of the lymphopOietic microenvironment and may be active participants in a complex, cytokine-mediated regulatory network.
Introduction of interleukin-3 gene into stromal cells from the bone marrow alters hemopoietic differentiation but does not modify stem cell renewal.
It is proposed that novel stromal cell factors, distinct from known CSFs, regulate stem cell renewal.
Myofibroblastic stromal cells isolated from human bone marrow induce the proliferation of both early myeloid and B-lymphoid cells.
Normal human bone marrow stromal cells isolated from Dexter-type long-term cultures are shown to induce the proliferation of both early myeloid and B-lymphoid cells in the absence of accessory hematopoietic cells and production of IL-7 appears to be a critical parameter that determines the ability of fibroblastic-like cells to induce BCP proliferation.
Myofibroblastic stromal cells isolated from human bone marrow induce the proliferation of both early myeloid and B-lymphoid cells
It is shown that normal human myofibroblastic BMSC induce the proliferation of both early myeloid and B-lymphoid cells in the absence of accessory hematopoietic cells.
Cytokine production and heterogeneity of primary stromal cells that support B lymphopoiesis
The results suggest that the primary stromal cells are a distinguishable cell type but functional subsets may exist, and the primary cell phenotype appears to mirror at least one division noted among theStromal cell lines.
Interleukin-4 induces a substance in bone marrow stromal cells that reversibly inhibits factor-dependent and factor-independent cell proliferation.
The capacity of IL-4 to induce inhibitory activity in stromal cell layers suggests that IL-2 may play a role in the regulation of hematopoiesis and partial inhibition could be achieved with pure macrophages stimulated with IL- 4 and CSF-1, suggesting that the inhibitoryActivity induced by IL-3 in mixed stroma cell layers may depend on a complex mechanism involving more than one cell type.
Native associations of early hematopoietic stem cells and stromal cells isolated in bone marrow cell aggregates.
A scheme of stromal cell-hematopoietic cell interactions based on the cell types selectively retained within the aggregates is proposed, which presents a novel means to study in vivo stem cell-stromal cell interaction.


Hematopoietic factor production by a cell line (TC-1) derived from adherent murine marrow cells.
An adherent cell line, termed TC-1, has been isolated from long-term liquid culture of murine marrow cells by repeated exposure of the adherent cells to 0.1% trypsin and its subclones may provide models for the control of early stem cell proliferation and differentiation.
Long-term culture of B lymphocytes and their precursors from murine bone marrow.
  • C. Whitlock, O. Witte
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1982
It is suggested that mature B cells arose via maturation of pre-B cells in the cultures that involved rearrangement and expression of different variable region gene segments.
Control of hemopoiesis by a bone marrow stromal cell clone: lipopolysaccharide- and interleukin-1-inducible production of colony-stimulating factors.
A stromal cell line, GY30, was cloned from mouse bone marrow adherent cell layers. In culture, GY30 cells sustain the production of granulocyte-macrophage progenitor cells (GM-CFU) but fail to
Differentiation stage and lineage-specific inhibitor from the stroma of mouse bone marrow that restricts lymphoma cell growth.
The high sensitivity of plasmacytomas to LCIA versus the resistance of normal stem cells may be utilized for selective elimination of plasma cell tumors from bone marrow inocula.
Corticosteroid-resistant bone marrow-derived B lymphocyte progenitor for long term in vitro cultures.
Results indicate that the cell responsible for the outgrowth of the long term B lineage cultures is corticosteroid resistant and is likely to be earlier in the B lymphocyte lineage than steroid-sensitive pre-B cells.
Lipid accumulation and production of colony-stimulating activity by the 266AD cell line derived from mouse bone marrow.
Growth of bone marrow cells in a collagen gel matrix provided a way to isolate stromal cells, and the 266AD cell line provides a means to examine the relationships betweenStromal cell lipogenesis and regulation of granulopoiesis.
Enrichment of hematopoietic precursor cells and cloning of multipotential B-lymphocyte precursors.
A simple one-step isolation technique significantly enriched mouse fetal liver cells that respond to interleukin 3 (IL-3), a multilineage hematopoietic growth factor, to maintain their capacity to differentiate to mature B lymphocytes committed to multiple Ig specificities.
Identification of a T cell-derived b cell growth factor distinct from interleukin 2
A factor found in induced supernatants of the mouse thymoma EL4 that co-stimulates with anti-IgM antibodies in short-term cultures of purified B lymphocytes to induce polyclonal B cell proliferation but not antibody-forming cell production is reported.
Role of the stromal cells in the regulation of granulopoiesis in long-term bone marrow culture: effects of conditioning medium on granulopoiesis "in vitro".
Mouse abdominal wall-conditioned medium contains a factor which stimulated granulocyte-monocyte colony formation, which suggests that granulopoiesis is controlled by intercellular interactions withStromal cells and that exogenous factors act only on the stem cells outside the stromal microenvironment.
Cultured mouse marrow stromal cell lines. II. Distinct subtypes differing in morphology, collagen types, myelopoietic factors, and leukemic cell growth modulating activities
A series of stromal cell lines were studied for their growth properties, electron microscopic morphology, cytochemical profile, collagen types, production of myelopoietic factors, and modulation of