A single bone marrow-derived stromal cell type supports the in vitro growth of early lymphoid and myeloid cells

@article{Hunt1987ASB,
  title={A single bone marrow-derived stromal cell type supports the in vitro growth of early lymphoid and myeloid cells},
  author={P. Hunt and D. Robertson and D. Weiss and D. Rennick and F. Lee and O. Witte},
  journal={Cell},
  year={1987},
  volume={48},
  pages={997-1007}
}
A clonal cell line (ALC) derived from murine bone marrow stroma is capable of supporting the continuous, in vitro growth of early lymphoid and myeloid cell populations. The growth-promoting effects of ALC are in part mediated through M-CSF and a pre-B cell growth factor, both of which accumulate in ALC-culture supernatant. To analyze the lymphoid growth factor produced by ALC cells, we derived a pre-B cell indicator line that is dependent on ALC-growth-conditioned medium. Using a combination of… Expand
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