A single assay for measuring the rates of phagocytosis and bacterial killing by neutrophils.

@article{Hampton1994ASA,
  title={A single assay for measuring the rates of phagocytosis and bacterial killing by neutrophils.},
  author={Mark B Hampton and Margret C. M. Vissers and Christine C Winterbourn},
  journal={Journal of leukocyte biology},
  year={1994},
  volume={55 2},
  pages={147-52}
}
We have developed a method that enables the rates of phagocytosis and killing of bacteria by neutrophils to be measured in a single assay. Neutrophils were incubated with bacteria, and at specific intervals were separated from uningested bacteria by low speed centrifugation. Rates of phagocytosis and killing were calculated from the decrease in number of extracellular bacteria and change in the number of intracellular bacteria. Both phagocytosis and killing were shown to follow first-order… CONTINUE READING