A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis

@article{Ozeki2006ASA,
  title={A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis},
  author={Johji Ozeki and Shuichiro Takahashi and Ken Komatsu and Satoshi Kagiwada and Kazuo Yamashita and Takuma Mori and Haruyuki Hirata and Yasuyuki Yamaji and Masashi Ugaki and Shigetou Namba},
  journal={Archives of Virology},
  year={2006},
  volume={151},
  pages={2067-2075}
}
From a lily isolate of Plantago asiatica mosaic virus (PlAMV-Li), two sub-isolates (Li1 and Li6) were obtained. Although the nucleotide sequences of Li1 and Li6 were highly conserved, they showed different pathogenicity in Nicotiana benthamiana. Li1 caused necrosis, whereas Li6 infected the plant asymptomatically. Inoculation tests with chimeric and point-mutated viruses revealed that amino acid 1154 of the RNA-dependent RNA polymerase (RdRp) contributes to the necrotic symptoms. The… CONTINUE READING