A simplified protein precipitation and filtration procedure for determining serum vitamin B6 by high-performance liquid chromatography.


Protein precipitation followed by centrifuge filtration was tested as a simplified sample preparation procedure for quantifying pyridoxal 5'-phosphate (PLP) and 4-pyridoxic acid (4PA) in serum by high-performance liquid chromatography. Serum samples (n=160) were prepared by both centrifuge filtration and an established technique using traditional… (More)
DOI: 10.1016/j.ab.2009.02.014

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