A simplified method for growth of human microvascular endothelial cells results in decreased senescence and continued responsiveness to cytokines and growth factors

@article{Krling1998ASM,
  title={A simplified method for growth of human microvascular endothelial cells results in decreased senescence and continued responsiveness to cytokines and growth factors},
  author={Birgit Maria Kr{\"a}ling and Joyce Bischoff},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  year={1998},
  volume={34},
  pages={308-315}
}
Human dermal microvascular endothelial cells are used to analyze the functions of microvascular endothelium in vitro. However, the low yield and short lifespan of these cells in culture has limited the types of analysis that could be performed. Human microvascular endothelial cells are typically grown in media containing supplements such as dibutyryl cyclic AMP, hydrocortisone, bovine brain extract, and antifungal agents, each of which increase the complexity of experimental design and… CONTINUE READING
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