A simplified method for freezing mouse blastocysts.


Mouse blastocysts frozen slowly (0.3-0.5 degrees C min-1) to -25 degrees C and transferred to -196 degrees C survive long-term storage to the extent comparable to unfrozen blastocysts. After thawing at 450 and 650 degrees C min-1, 91 and 93% blastocysts, respectively, continued normal development under in vitro conditions. The length of equilibration at -25… (More)


Cite this paper

@article{Landa1982ASM, title={A simplified method for freezing mouse blastocysts.}, author={Vladim{\'i}r Landa}, journal={Folia biologica}, year={1982}, volume={28 4}, pages={266-73} }