A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections.

@article{Newton2002ASM,
  title={A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections.},
  author={Sathyanesan Samuel Newton and Antonia Dow and Rose Marie Z. Terwilliger and Ronald C Duman},
  journal={Brain research. Brain research protocols},
  year={2002},
  volume={9 3},
  pages={214-9}
}
A method is described to perform combined immunohistochemistry and in situ hybridization in mouse brain sections. The protocol is specific to sections mounted on glass slides. In contrast to earlier methods that require either paraffin embedding or perfusion of the brain with paraformaldehyde, this protocol can be carried out on fresh-frozen, cryostat cut post-fixed sections. This simple and concise protocol increases the applicability of the technique as the RNAse-free immunodetection of… CONTINUE READING

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