A simplified duplex real-time PCR incorporating TaqMan minor groove binder ( MGB ) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures

@inproceedings{EhtishamulHaque2015ASD,
  title={A simplified duplex real-time PCR incorporating TaqMan minor groove binder ( MGB ) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures},
  author={Syed Ehtisham-ul-Haque and Sajjad ur Rahman and Mazhar I. Khan and Muhammad Younus and Mian M. Awais and Asghar Nasir},
  year={2015}
}
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most pathogenic and economically important mycoplasma pathogens that infect chickens. The development of rapid and innovative molecular diagnostic techniques is of pivotal importance for their effective control. The aim of the present study was to develop a novel duplex TaqMan real-time PCR assay for the simultaneous detection of MG and MS. This duplex real-time PCR assay incorporates TaqMan (FAM/NED) labelled minor groove… CONTINUE READING

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