A simplified and standardized polymerase chain reaction format for the diagnosis of leishmaniasis.

@article{Deborggraeve2008ASA,
  title={A simplified and standardized polymerase chain reaction format for the diagnosis of leishmaniasis.},
  author={Stijn Deborggraeve and Thierry Laurent and Diego A Espinosa and Gert Van der Auwera and Margaret W Mbuchi and Monique K. Wasunna and Sayda El-Safi and Ahmed Almustafa Al-Basheer and Jorge Luis S{\'a}nchez Ar{\'e}valo and C{\'e}sar Miranda-Ver{\'a}stegui and Thierry Leclipteux and Pascal G N Mertens and J{\'e}r{\^o}me Dujardin and Piet Herdewijn and Philippe B{\"u}scher},
  journal={The Journal of infectious diseases},
  year={2008},
  volume={198 10},
  pages={
          1565-72
        }
}
BACKGROUND Definite diagnosis of Leishmania infections is based on demonstration of the parasite by microscopic analysis of tissue biopsy specimens or aspirate samples. However, microscopy generally shows low sensitivity and requires invasive sampling. METHODS We describe here the development of a simple and rapid test for the detection of polymerase chain reaction-amplified Leishmania DNA. A phase 1 evaluation of the text was conducted in clinical samples from 60 nonendemic and 45 endemic… CONTINUE READING

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