A simplified Timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain.

@article{Sloviter1982AST,
  title={A simplified Timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain.},
  author={Robert S. Sloviter},
  journal={Brain research bulletin},
  year={1982},
  volume={8 6},
  pages={771-4}
}
A simplified sulphide/silver technique for visualizing transitional metals in rat brain is described. It consists of: (1) sulphide perfusion before formaldehyde perfusion, (2) routine paraffin embedding and sectioning, and (3) silver staining with physical developer. This method is compatible with formaldehyde fixation, eliminates the need for post-fixation in Carnoy's solution before embedding and does not require low paraffin temperatures during embedding. Once embedded, brains can be stored… CONTINUE READING

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