A simple stopped assay for fatty acid amide hydrolase avoiding the use of a chloroform extraction phase.

@article{Boldrup2004ASS,
  title={A simple stopped assay for fatty acid amide hydrolase avoiding the use of a chloroform extraction phase.},
  author={Linda Boldrup and Sandy J Wilson and Ann J Barbier and Christopher J Fowler},
  journal={Journal of biochemical and biophysical methods},
  year={2004},
  volume={60 2},
  pages={171-7}
}
A stopped assay for fatty acid amide hydrolase (FAAH) has been developed, whereby the enzyme reaction product ([(3)H]ethanolamine) was separated from substrate (anandamide [ethanolamine-1-(3)H]), by differential adsorption to charcoal. The assay gave a better extraction efficiency when acidic rather than alkaline charcoal solutions were used to stop the… CONTINUE READING