A simple selection strategy for evolving highly efficient enzymes

Abstract

Combining tunable transcription with an enzyme-degradation tag affords an effective means to reduce intracellular enzyme concentrations from high to very low levels. Such fine-tuned control allows selection pressure to be systematically increased in directed-evolution experiments. This facilitates identification of mutants with wild-type activity, as shown… (More)
DOI: 10.1038/nbt1341

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Cite this paper

@article{Neuenschwander2007ASS, title={A simple selection strategy for evolving highly efficient enzymes}, author={Martin Neuenschwander and Maren Butz and Caroline Heintz and Peter Kast and Donald Hilvert}, journal={Nature Biotechnology}, year={2007}, volume={25}, pages={1145-1147} }