A simple screen for murein transglycosylase inhibitors.

  title={A simple screen for murein transglycosylase inhibitors.},
  author={Waldemar Vollmer and J V H{\"o}ltje},
  journal={Antimicrobial agents and chemotherapy},
  volume={44 5},
A simple assay for detection of compounds that bind to the active site in the transglycosylation domain of the essential bifunctional transglycosylase and transpeptidase penicillin-binding proteins (PBPs) is reported. The method is based on a competition with the specific transglycosylase inhibitor moenomycin. With moenomycin coupled to Affi-Gel beads, a simple filtration procedure allows the amount of labeled PBPs that bind to moenomycin beads in the presence of test substances to be… CONTINUE READING