Corpus ID: 25336129

A simple quantitative in vitro macrophage migration assay.

  title={A simple quantitative in vitro macrophage migration assay.},
  author={Kamlesh K. Bhopale and Kaori Masani and K. S. Pradhan and Chaman Lal Kaul},
  journal={Indian journal of experimental biology},
  volume={34 10},
An in vitro macrophage chemotaxis model using mouse peritoneal non-elicited resident macrophage cells and chemotaxins containing mediators of non-specific elicitors such as oyster glycogen or sodium caseinate has been described. Macrophage cells accumulation in mouse peritoneal cavity was maximum at 48 hr after injecting (i.p.) oyster glycogen (2.5%) or sodium caseinate (12%), 0.5 ml/mouse. Chemotaxins containing mediators were prepared from these mice by peritoneal lavage and termed as routine… Expand

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