A simple protocol for paraffin‐embedded myelin sheath staining with osmium tetroxide for light microscope observation

@article{Scipio2008ASP,
  title={A simple protocol for paraffin‐embedded myelin sheath staining with osmium tetroxide for light microscope observation},
  author={Federica Di Scipio and Stefania Raimondo and Pierluigi Tos and Stefano Geuna},
  journal={Microscopy Research and Technique},
  year={2008},
  volume={71}
}
Experimental investigation of peripheral nerve fiber regeneration is attracting more and more attention among both basic and clinical researchers. Assessment of myelinated nerve fiber morphology is a pillar of peripheral nerve regeneration research. The gold standard for light microscopic imaging of myelinated nerve fibers is toluidine blue staining of resin‐embedded semithin sections. However, many researchers are unaware that the dark staining of myelin sheaths typically produced by this… Expand
Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology.
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The intent of this publication is to accentuate written protocols for toluidine blue staining of peripheral nerves with videography of the method, using sciatic nerves harvested from rats. Expand
Staining Methods for Normal and Regenerative Myelin in the Nervous System.
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Three histochemical methods for the staining of myelin suitable for formalin-fixed and paraffin-embedded materials are described and the osmium tetroxide method is described, which consist in the Osmication of previously fixed tissues. Expand
A Rapid Protocol for Intraoperative Assessment of Peripheral Nerve Myelinated Axon Count and Its Application to Cross-Facial Nerve Grafting
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A rapid protocol for quantification of myelinated axon counts from peripheral nerves using widely available equipment and techniques has been described, rendering possible intraoperative assessment of donor nerve suitability for reanimation. Expand
Evaluation of myelin sheath and collagen reorganization pattern in a model of peripheral nerve regeneration using an integrated histochemical approach
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Comparisons of the three fixation techniques for the assessment of peripheral nerve morphology revealed that normal myelinated axons were markedly lower in the immersion fixation group compared to those of instillation and transcardial perfusion. Expand
Chapter 5: Methods and protocols in peripheral nerve regeneration experimental research: part II-morphological techniques.
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The importance of osmium tetroxide post-fixation as a useful procedure to be adopted independently from the embedding medium is emphasized and the basic rules of morpho-quantitative stereological analysis of nerve fibers are described addressing the important concepts of design-based sampling and the disector. Expand
COMPARISON OF HEMATOXYLIN AND EOSIN STAINING WITH AND WITHOUT PRE TREATMENT WITH MARCHI ’ S SOLUTION ON NERVE SAMPLES FOR NERVE DEGENERATION AND REGENERATION STUDIES
The study was conducted on four healthy guinea pigs ( Cavia porcellus) of either sex in which the nerve was identified and subjected to crush injury with the tip (3mm) of a curved hemostatic forceps.Expand
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TLDR
This work shows a method of visualizing myelin that is fast, inexpensive and reliable using the cross-linking fixative glutaraldehyde that produces strong, broad-spectrum auto-fluorescence in fixed tissue and shows the feasibility of immunohistochemistry with antigen retrieval, which allows co-localization of proteins of interest together with detailed myelin morphology. Expand
NERVE FIBER DIAMETER MEASUREMENTS USING HEMATOXYLIN AND EOSIN STAINING AND BRIGHTFIELD MICROSCOPY TO ASSESS THE NOVEL METHOD OF CHARACTERIZING PERIPHERAL NERVE FIBER DISTRIBUTIONS BY GROUP DELAY
Peripheral neuropathies are a set of common diseases that affect the peripheral nervous system, causing damage to vital connections between various parts of the body and the brain and spinal cord.Expand
A simple and robust method for automating analysis of naïve and regenerating peripheral nerves
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Automatic and automatic ADS axon counts demonstrated good agreement in naïve nerves and moderate agreement on regenerating nerves, and ADS is an accessible and robust tool for AH that can provide consistent analysis across protocols and nerve states. Expand
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