A simple procedure for the extraction of DNA from long-term formalin-preserved brain tissues for the detection of EBV by PCR.

@article{Hassani2015ASP,
  title={A simple procedure for the extraction of DNA from long-term formalin-preserved brain tissues for the detection of EBV by PCR.},
  author={Asma Hassani and Gulfaraz Khan},
  journal={Experimental and molecular pathology},
  year={2015},
  volume={99 3},
  pages={558-63}
}
Long-term formalin fixed brain tissues are potentially an important source of material for molecular studies. Ironically, very few protocols have been published describing DNA extraction from such material for use in PCR analysis. In our attempt to investigate the role of Epstein-Barr virus (EBV) in the pathogenesis of multiple sclerosis (MS), extracting PCR quality DNA from brain samples fixed in formalin for 2-22 years, proved to be very difficult and challenging. As expected, DNA extracted… CONTINUE READING
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