A simple procedure for the analysis of single nucleotide polymorphisms facilitates map-based cloning in Arabidopsis.

@article{Drenkard2000ASP,
  title={A simple procedure for the analysis of single nucleotide polymorphisms facilitates map-based cloning in Arabidopsis.},
  author={Eliana Drenkard and Brent G. Richter and Steve Rozen and Lisa M Stutius and N A Angell and Michael Mindrinos and Raymond J. Cho and Peter J. Oefner and Ronald W. Davis and Frederick M. Ausubel},
  journal={Plant physiology},
  year={2000},
  volume={124 4},
  pages={1483-92}
}
We developed a modified allele-specific PCR procedure for assaying single nucleotide polymorphisms (SNPs) and used the procedure (called SNAP for single-nucleotide amplified polymorphisms) to generate 62 Arabidopsis mapping markers. SNAP primers contain a single base pair mismatch within three nucleotides from the 3' end of one allele (the specific allele) and in addition have a 3' mismatch with the nonspecific allele. A computer program called SNAPER was used to facilitate the design of… CONTINUE READING
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