A simple procedure for protein ubiquitination detection in Saccharomyces cerevisiae: Gap1p as an example.

@article{Lv2013ASP,
  title={A simple procedure for protein ubiquitination detection in Saccharomyces cerevisiae: Gap1p as an example.},
  author={Yongkun Lv and Xinrui Zhao and Long Liu and Guocheng Du and Jingwen Zhou and Jian Chen},
  journal={Journal of microbiological methods},
  year={2013},
  volume={94 1},
  pages={25-9}
}
We established a simple procedure for protein ubiquitination detection in Saccharomyces cerevisiae. Enhanced green fluorescent protein (EGFP) was split into two parts, an N-terminal (GN) and a C-terminal (GC) region. The fusion fragments GN-UBI3 and multi-cloning site (MCS)-GC were inserted into the vector pY26-TEF/GPD, resulting in pUbDetec16. pUbDetec16 was designed for use in detecting protein ubiquitination. Any gene of interest can be inserted into the MCS and the recombinant plasmid can… CONTINUE READING
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