A simple polypyrimidine repeat acts as an artificial Rho-dependent terminator in vivo and in vitro.

@article{Guerin1998ASP,
  title={A simple polypyrimidine repeat acts as an artificial Rho-dependent terminator in vivo and in vitro.},
  author={Megan Guerin and No el Robichon and Johannes Geiselmann and Abdelkader Rahmouni},
  journal={Nucleic acids research},
  year={1998},
  volume={26 21},
  pages={4895-900}
}
In this paper, we present evidence that an efficient Rho-dependent terminator can be created by introducing a simple (AG/TC) n DNA repeat into a transcription unit. The Rho termination activity in vivo and in vitro is dependent on the length and the orientation of the insert. The transcription of at least 30 bp of the (AG/TC) n repeat in the orientation encoding the (rUrC) n sequence on the transcript leads to Rho-dependent termination at a downstream non-terminator site. Our results indicate… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.
10 Extracted Citations
0 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 10 extracted citations

Similar Papers

Loading similar papers…