A simple perfusion technique for isolation of maternal intervillous blood mononuclear cells from human placentae.

@article{Moore1997ASP,
  title={A simple perfusion technique for isolation of maternal intervillous blood mononuclear cells from human placentae.},
  author={Julie M. Moore and Bernard L. Nahlen and Ayub V. O. Ofulla and Joseph Caba and John G. Ayisi and A. Mayabi Oloo and Ambrose O. Misore and Andre Joseph Nahmias and Altaf A. Lal and Venkatachalam Udhayakumar},
  journal={Journal of immunological methods},
  year={1997},
  volume={209 1},
  pages={93-104}
}
A noninvasive perfusion method for the recovery of maternal placental (intervillous) blood for use in immunologic assays is described. 60% of the perfused blood samples tested for fetal red blood cell (RBC) contamination were found to be pure maternal blood; in the remainder, fetal RBC contamination, with a single exception, was less than 6%. The intervillous mononuclear cells (IVBMC) isolated from this blood were of predominantly maternal origin as demonstrated by a polymerase chain reaction… CONTINUE READING