A simple multiplex polymerase chain reaction to determine ABO blood types of rhesus macaques (Macaca mulatta).

@article{Premasuthan2011ASM,
  title={A simple multiplex polymerase chain reaction to determine ABO blood types of rhesus macaques (Macaca mulatta).},
  author={A. Premasuthan and Sree Kanthaswamy and Jessica A Satkoski and D. G. Smith},
  journal={Tissue antigens},
  year={2011},
  volume={77 6},
  pages={
          584-8
        }
}
Rhesus macaques are the most common nonhuman primate model organism used in biomedical research. Their increasingly frequent use as subjects in studies involving transplantation requires that blood and other tissue antigens of donors and recipients be compatible. We report here an easy and rapid multiplex polymerase chain reaction (PCR) to determine the ABO blood group phenotypes of rhesus macaques that can be performed with only small amounts of DNA. We phenotyped 78 individuals and found this… 
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TLDR
A novel method to detect the A, B, and AB phenotypes of macaques using real-time quantitative polymerase chain reaction is developed, which enables the simple and rapid screening of these phenotypes in macaques without the need for fresh blood or saliva.
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TLDR
This work compared the ability of two established ABO-typing methods, namely, serological testing and immunohistochemistry, and a novel polymerase chain reaction (PCR)-based assay to type 66 rhesus monkeys, and found that the IHC and PCR unequivocally yielded the same types in all monkeys.
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TLDR
A more expansive model of hybridization between rhesus and cynomolgus macaques than often proposed is supported and highlights the increased need for consideration of population genetic structure in biomedical studies that employ macaques as animal models.
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TLDR
The close similarity in blood group frequency distributions between the Chinese rhesus and Indochinese cynomolgus macaques demonstrates that the introgression between these two species extends beyond the zone of intergradation in Indochina.
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TLDR
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TLDR
It is hypothesized, albeit without molecular confirmation, that the indeterminate phenotype represents homozygosity for the null O allele at the macaque ABO locus, and may represent the unsuccessful detection of either A or B alleles using primers targeting the A‐specific and B‐specific single nucleotide polymorphisms (SNPs) in a variable region of exon 7 of the A BO locus.
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