A simple molecular method for monitoring co-inoculated yeasts during wine fermentation

  title={A simple molecular method for monitoring co-inoculated yeasts during wine fermentation},
  author={Carla Jara and Alejandra Garc{\'i}a Rojas and Jaime Romero},
  journal={Annals of Microbiology},
The inoculation of Torulaspora into wine is an increasingly popular strategy for improving aromatic profiles. However, questions remain regarding the use of this yeast for alcoholic fermentation (AF). This work presents a simple method based on a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of internal transcribed spacers (ITSs) that can be used to distinguish between Torulaspora and Saccharomyces, the most commonly co-inoculated yeasts in wines. 
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